{"id":5604,"date":"2017-12-06T12:30:59","date_gmt":"2017-12-06T12:30:59","guid":{"rendered":"http:\/\/www.birds.cornell.edu\/ccb\/?p=5604"},"modified":"2021-06-09T19:59:16","modified_gmt":"2021-06-09T19:59:16","slug":"studying-a-cryptic-marine-mammal-species-by-edna","status":"publish","type":"post","link":"https:\/\/www.birds.cornell.edu\/ccb\/studying-a-cryptic-marine-mammal-species-by-edna\/","title":{"rendered":"Studying a cryptic marine mammal species by eDNA"},"content":{"rendered":"\n<div class=\"wp-block-image\"><figure class=\"alignright size-medium\"><a href=\"http:\/\/www.birds.cornell.edu\/ccb\/wp-content\/uploads\/2017\/11\/HICEAS_LJ_Blog.png\"><img decoding=\"async\" width=\"720\" height=\"473\" data-src=\"https:\/\/www.birds.cornell.edu\/ccb\/wp-content\/uploads\/2017\/11\/HICEAS_LJ_Blog-e1512142607138-720x473.png\" alt=\"R\/V Reuben Lasker at the port\" class=\"wp-image-5657 lazyload\" data-srcset=\"https:\/\/www.birds.cornell.edu\/ccb\/wp-content\/uploads\/2017\/11\/HICEAS_LJ_Blog-e1512142607138-720x473.png 720w, https:\/\/www.birds.cornell.edu\/ccb\/wp-content\/uploads\/2017\/11\/HICEAS_LJ_Blog-e1512142607138-768x504.png 768w, https:\/\/www.birds.cornell.edu\/ccb\/wp-content\/uploads\/2017\/11\/HICEAS_LJ_Blog-e1512142607138-1280x841.png 1280w, https:\/\/www.birds.cornell.edu\/ccb\/wp-content\/uploads\/2017\/11\/HICEAS_LJ_Blog-e1512142607138-1920x1261.png 1920w, https:\/\/www.birds.cornell.edu\/ccb\/wp-content\/uploads\/2017\/11\/HICEAS_LJ_Blog-e1512142607138-480x315.png 480w\" data-sizes=\"(max-width: 720px) 100vw, 720px\" src=\"data:image\/svg+xml;base64,PHN2ZyB3aWR0aD0iMSIgaGVpZ2h0PSIxIiB4bWxucz0iaHR0cDovL3d3dy53My5vcmcvMjAwMC9zdmciPjwvc3ZnPg==\" style=\"--smush-placeholder-width: 720px; --smush-placeholder-aspect-ratio: 720\/473;\" \/><\/a><figcaption>The R\/V Reuben Lasker in port at Ford Island, Honolulu<\/figcaption><\/figure><\/div>\n\n\n\n<p class=\"intro\">In the words of The Who, I am reminded of a catchy tune: \u201cWho are you? Who, who, who, who?\u201d As we search for the identity of a mysterious beaked whale, I just can\u2019t help but find this to be the most fitting theme song for my expedition to the central Pacific Ocean. In mid-October, I had the exciting opportunity to participate in the <a href=\"https:\/\/www.pifsc.noaa.gov\/hiceas\/\" target=\"_blank\" rel=\"noopener\">Hawaiian Islands Cetacean Assessment and Ecosystem Survey (HICEAS)<\/a> aboard NOAA&#8217;s R\/V Reuben Lasker. This was a special opportunity, not only because it was my first pelagic research cruise, but I also had the chance to participate in a study using environmental DNA (eDNA) to learn more about a cryptic marine mammal species.<\/p>\n\n\n\n<p class=\"intro\">I spent a total of 25 days at sea, but the entire HICEAS effort will span a total of 187 days aboard two NOAA ships: the <a href=\"https:\/\/www.omao.noaa.gov\/learn\/marine-operations\/ships\/oscar-elton-sette\" target=\"_blank\" rel=\"noopener\">R\/V Oscar Elton Sette<\/a> and the <a href=\"https:\/\/www.omao.noaa.gov\/learn\/marine-operations\/ships\/reuben-lasker\" target=\"_blank\" rel=\"noopener\">R\/V Reuben Lasker<\/a>. The HICEAS expedition was a huge collaborative effort to study whales, dolphins, seabirds, and the ecosystem of the Pacific Ocean around the Main and Northwest Hawaiian Islands. Both ships made multiple trips, or \u2018legs\u2019 to cover the entire Island territory; my involvement was on leg 3 of 4 on the R\/V Reuben Lasker. We departed from Oahu and traveled west along different transect lines on the north side of the Northwest Hawaiian Islands out to Midway Island. We circumnavigated Midway, and then returned to Oahu by way of the south side of the Island chain. Plans to cross the International Date Line were thwarted by rough seas, which you can see in the photo. In general, HICEAS (oops, I mean high seas) were a common theme for the trip; we averaged&nbsp;6-10 foot seas and 25 knot winds for the entire leg.<\/p>\n\n\n\n<figure class=\"wp-block-gallery columns-3 is-cropped wp-block-gallery-1 is-layout-flex wp-block-gallery-is-layout-flex\"><ul class=\"blocks-gallery-grid\"><li class=\"blocks-gallery-item\"><figure><img decoding=\"async\" width=\"1280\" height=\"777\" data-src=\"https:\/\/www.birds.cornell.edu\/ccb\/wp-content\/uploads\/2017\/11\/Photo2_regised-1280x777.png\" alt=\"\" data-id=\"5660\" data-full-url=\"https:\/\/www.birds.cornell.edu\/ccb\/wp-content\/uploads\/2017\/11\/Photo2_regised.png\" data-link=\"https:\/\/www.birds.cornell.edu\/ccb\/studying-a-cryptic-marine-mammal-species-by-edna\/photo2_regised\/\" class=\"wp-image-5660 lazyload\" data-srcset=\"https:\/\/www.birds.cornell.edu\/ccb\/wp-content\/uploads\/2017\/11\/Photo2_regised-1280x777.png 1280w, https:\/\/www.birds.cornell.edu\/ccb\/wp-content\/uploads\/2017\/11\/Photo2_regised-720x437.png 720w, https:\/\/www.birds.cornell.edu\/ccb\/wp-content\/uploads\/2017\/11\/Photo2_regised-768x466.png 768w, https:\/\/www.birds.cornell.edu\/ccb\/wp-content\/uploads\/2017\/11\/Photo2_regised-1920x1165.png 1920w, https:\/\/www.birds.cornell.edu\/ccb\/wp-content\/uploads\/2017\/11\/Photo2_regised-480x291.png 480w\" data-sizes=\"(max-width: 1280px) 100vw, 1280px\" src=\"data:image\/svg+xml;base64,PHN2ZyB3aWR0aD0iMSIgaGVpZ2h0PSIxIiB4bWxucz0iaHR0cDovL3d3dy53My5vcmcvMjAwMC9zdmciPjwvc3ZnPg==\" style=\"--smush-placeholder-width: 1280px; --smush-placeholder-aspect-ratio: 1280\/777;\" \/><figcaption class=\"blocks-gallery-item__caption\">Sunrise over Midway Island<\/figcaption><\/figure><\/li><li class=\"blocks-gallery-item\"><figure><img decoding=\"async\" width=\"1280\" height=\"777\" data-src=\"https:\/\/www.birds.cornell.edu\/ccb\/wp-content\/uploads\/2017\/11\/Photo3_Revised-1280x777.png\" alt=\"\" data-id=\"5662\" data-full-url=\"https:\/\/www.birds.cornell.edu\/ccb\/wp-content\/uploads\/2017\/11\/Photo3_Revised.png\" data-link=\"https:\/\/www.birds.cornell.edu\/ccb\/studying-a-cryptic-marine-mammal-species-by-edna\/photo3_revised\/\" class=\"wp-image-5662 lazyload\" data-srcset=\"https:\/\/www.birds.cornell.edu\/ccb\/wp-content\/uploads\/2017\/11\/Photo3_Revised-1280x777.png 1280w, https:\/\/www.birds.cornell.edu\/ccb\/wp-content\/uploads\/2017\/11\/Photo3_Revised-720x437.png 720w, https:\/\/www.birds.cornell.edu\/ccb\/wp-content\/uploads\/2017\/11\/Photo3_Revised-768x466.png 768w, https:\/\/www.birds.cornell.edu\/ccb\/wp-content\/uploads\/2017\/11\/Photo3_Revised-1920x1165.png 1920w, https:\/\/www.birds.cornell.edu\/ccb\/wp-content\/uploads\/2017\/11\/Photo3_Revised-480x291.png 480w\" data-sizes=\"(max-width: 1280px) 100vw, 1280px\" src=\"data:image\/svg+xml;base64,PHN2ZyB3aWR0aD0iMSIgaGVpZ2h0PSIxIiB4bWxucz0iaHR0cDovL3d3dy53My5vcmcvMjAwMC9zdmciPjwvc3ZnPg==\" style=\"--smush-placeholder-width: 1280px; --smush-placeholder-aspect-ratio: 1280\/777;\" \/><figcaption class=\"blocks-gallery-item__caption\">High seas on the HICEAS<\/figcaption><\/figure><\/li><\/ul><\/figure>\n\n\n\n<div class=\"wp-block-image\"><figure class=\"alignright\"><a href=\"http:\/\/www.birds.cornell.edu\/ccb\/wp-content\/uploads\/2017\/11\/Photo-4-ab.png\"><img decoding=\"async\" width=\"720\" height=\"514\" data-src=\"http:\/\/www.birds.cornell.edu\/ccb\/wp-content\/uploads\/2017\/11\/Photo-4-ab-720x514.png\" alt=\"Lauren deploying a sonobuoy aboard the R\/V Reuben Lasker\" class=\"wp-image-5671 lazyload\" data-srcset=\"https:\/\/www.birds.cornell.edu\/ccb\/wp-content\/uploads\/2017\/11\/Photo-4-ab-720x514.png 720w, https:\/\/www.birds.cornell.edu\/ccb\/wp-content\/uploads\/2017\/11\/Photo-4-ab-768x549.png 768w, https:\/\/www.birds.cornell.edu\/ccb\/wp-content\/uploads\/2017\/11\/Photo-4-ab-1280x914.png 1280w, https:\/\/www.birds.cornell.edu\/ccb\/wp-content\/uploads\/2017\/11\/Photo-4-ab-1920x1371.png 1920w, https:\/\/www.birds.cornell.edu\/ccb\/wp-content\/uploads\/2017\/11\/Photo-4-ab-480x343.png 480w\" data-sizes=\"(max-width: 720px) 100vw, 720px\" src=\"data:image\/svg+xml;base64,PHN2ZyB3aWR0aD0iMSIgaGVpZ2h0PSIxIiB4bWxucz0iaHR0cDovL3d3dy53My5vcmcvMjAwMC9zdmciPjwvc3ZnPg==\" style=\"--smush-placeholder-width: 720px; --smush-placeholder-aspect-ratio: 720\/514;\" \/><\/a><figcaption>Lauren deploying a sonobuoy aboard the R\/V Reuben Lasker<\/figcaption><\/figure><\/div>\n\n\n\n<p class=\"intro\">Despite the lack of cooperation from Mother Nature, we managed to see a lot of wildlife! I learned so much about sea birds! I saw 5 different types of boobies, tons of Bonin petrels, wedge-tailed shearwaters, plovers, white terns, and black-footed albatross. In my opinion, observing bird behavior was&nbsp;one of the most interesting parts of the trip. On one occasion, I witnessed a fascinating kleptoparasitic duet. Two Bonin petrels harassed a storm petrel to give up its meal. When the first thief stole the fish, the second Bonin petrel harassed the first one to give up the loot. I\u2019m glad that I have better friends than that! We saw several large groups of sperm whales, most of which were cow-calf nurseries. We also saw several Fraser\u2019s dolphins swimming in multiple chorus lines, Blainville\u2019s beaked whales, pygmy sperm whales, false killer whales, pilot whales, and humpback whales. I found it particularly interesting to monitor the acoustics equipment used on this trip. We towed an acoustics array, which detected high frequency sounds, deployed sonobuoys in the evenings to study low frequency calls, and operated an echosounder to monitor fish, squid, and crustacean populations. Tracking minke whale &#8220;boings,&#8221; dolphin whistles in real time, and clicks from the elusive \u2018Cross Seamount beaked whale\u2019 were highlights for me.<\/p>\n\n\n\n<div class=\"wp-block-image\"><figure class=\"aligncenter\"><a href=\"http:\/\/www.birds.cornell.edu\/ccb\/wp-content\/uploads\/2017\/11\/eDNA_LJ_One.jpg\"><img decoding=\"async\" width=\"1280\" height=\"707\" data-src=\"http:\/\/www.birds.cornell.edu\/ccb\/wp-content\/uploads\/2017\/11\/eDNA_LJ_One-e1512146844333-1280x707.jpg\" alt=\"Laboratory set up of filtering equipment\" class=\"wp-image-5643 lazyload\" data-srcset=\"https:\/\/www.birds.cornell.edu\/ccb\/wp-content\/uploads\/2017\/11\/eDNA_LJ_One-e1512146844333-1280x707.jpg 1280w, https:\/\/www.birds.cornell.edu\/ccb\/wp-content\/uploads\/2017\/11\/eDNA_LJ_One-e1512146844333-720x398.jpg 720w, https:\/\/www.birds.cornell.edu\/ccb\/wp-content\/uploads\/2017\/11\/eDNA_LJ_One-e1512146844333-768x424.jpg 768w, https:\/\/www.birds.cornell.edu\/ccb\/wp-content\/uploads\/2017\/11\/eDNA_LJ_One-e1512146844333-1920x1061.jpg 1920w, https:\/\/www.birds.cornell.edu\/ccb\/wp-content\/uploads\/2017\/11\/eDNA_LJ_One-e1512146844333-480x265.jpg 480w\" data-sizes=\"(max-width: 1280px) 100vw, 1280px\" src=\"data:image\/svg+xml;base64,PHN2ZyB3aWR0aD0iMSIgaGVpZ2h0PSIxIiB4bWxucz0iaHR0cDovL3d3dy53My5vcmcvMjAwMC9zdmciPjwvc3ZnPg==\" style=\"--smush-placeholder-width: 1280px; --smush-placeholder-aspect-ratio: 1280\/707;\" \/><\/a><figcaption>Laboratory set up of filtering equipment<\/figcaption><\/figure><\/div>\n\n\n\n<p class=\"intro\">My primary duty on leg 3 of the R\/V Reuben Lasker was to participate in a project to study the mysterious \u2018Cross Seamount beaked whale.\u2019 At Cross Seamount, which is located approximately 150 nautical miles south of Oahu, unique beaked whale vocalizations have been recorded but they have yet to be associated with a known species. Of the <span style=\"color: #000000;\">approximately <\/span>75 different whale, dolphin, and porpoise species that make click-type noises (echolocation), scientists have taxonomically narrowed this animal down to 1 of 4 types of beaked whales. The animal\u2019s true identity has yet to be determined, so it has been affectionately referred to as the \u2018Cross Seamount beaked whale.\u2019 Since beaked whales are generally difficult to see (even in low sea states) and these calls occur primarily at night, sighting data are scarce. Extracting eDNA from seawater&nbsp;offers an&nbsp;opportunity to collect biologic&nbsp;information from&nbsp;the animal generating these calls.<\/p>\n\n\n\n<div class=\"wp-block-image\"><figure class=\"alignright size-full\"><a href=\"http:\/\/www.birds.cornell.edu\/ccb\/wp-content\/uploads\/2017\/11\/filtering-eDNA_edit.png\"><img decoding=\"async\" width=\"466\" height=\"541\" data-src=\"https:\/\/www.birds.cornell.edu\/ccb\/wp-content\/uploads\/2017\/11\/filtering-eDNA_edit-e1512147263311.png\" alt=\"Lauren and (hopefully) some beaked whale DNA\" class=\"wp-image-5641 lazyload\" src=\"data:image\/svg+xml;base64,PHN2ZyB3aWR0aD0iMSIgaGVpZ2h0PSIxIiB4bWxucz0iaHR0cDovL3d3dy53My5vcmcvMjAwMC9zdmciPjwvc3ZnPg==\" style=\"--smush-placeholder-width: 466px; --smush-placeholder-aspect-ratio: 466\/541;\" \/><\/a><figcaption>Lauren and (hopefully) some beaked whale DNA<\/figcaption><\/figure><\/div>\n\n\n\n<p class=\"intro\">In efforts to identify the cryptic \u2018Cross Seamount beaked whale,\u2019 we collected water samples at several depths in places where positive acoustic detections were made. Water samples were collected at the surface and at a range of depths (0&#8211;1,000 meters deep) with a CTD (conductivity-temperature-depth) unit, which is an oceanographic instrument used to track ocean currents. Water was then frozen and filtered through a polycarbonate filter to concentrate the DNA. In total, we collected over 170 liters of water at different sites and depths, all of which were&nbsp;filtered during my trip. Once the seawater was reduced on the&nbsp;round filter paper, the paper was folded and spiraled into an ice cream cone-like shape, and placed into a microtube. A preservative called Longmire&#8217;s&nbsp;solution was added for storage and later transport.<span style=\"color: #000000;\">&nbsp;After docking, the samples will be sent to the <a href=\"https:\/\/mmi.oregonstate.edu\/ccgl\" target=\"_blank\" rel=\"noopener\">Cetacean Conservation and Genomics Lab at Oregon State University <\/a><\/span>to be analyzed by Dr. Scott Baker and his team to investigate the genetic material in these samples. Check out <a href=\"http:\/\/www.dna-surveillance.auckland.ac.nz\/\" target=\"_blank\" rel=\"noopener\">DNA Surveillance <\/a>to learn more.These methods have been successful for identifying other marine mammal species, so stay tuned to see what we will find! \u201cWho are you,\u201d beaked whale? \u201cWho, who, who, who?\u201d<\/p>\n\n\n\n<p class=\"intro\">If you are interested in following the remaining cruise or looking through all the exciting things that have happened on the HICEAS&nbsp;efforts, click <a href=\"https:\/\/www.pifsc.noaa.gov\/hiceas\/\" target=\"_blank\" rel=\"noopener\">here.<\/a><\/p>\n\n\n\n<p class=\"intro\"><em>Acknowledgements:&nbsp;Lauren&#8217;s trip was funded by the Office of Naval Research and made possible by the collaborative efforts of Pacific Island Fisheries Science Center (PIFSC), Southwest Fisheries Science Center (SWFSC), Cornell University, and Oregon State University.<\/em><\/p>\n","protected":false},"excerpt":{"rendered":"<p>In the words of The Who, I am reminded of a catchy tune: \u201cWho are you? Who, who, who, who?\u201d As we search for the identity of a mysterious beaked whale, I just can\u2019t help but find this to be the most fitting theme song for my expedition to the central Pacific Ocean. In mid-October,<a class=\"excerpt-read-more\" href=\"https:\/\/www.birds.cornell.edu\/ccb\/studying-a-cryptic-marine-mammal-species-by-edna\/\" title=\"ReadStudying a cryptic marine mammal species by eDNA\">&#8230; Read more &raquo;<\/a><\/p>\n","protected":false},"author":2,"featured_media":5717,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_birdpress_hero_toggle":false,"_birdpress_hero_type":"image","_birdpress_hero_image_type":"image","_birdpress_hero_style":"default","_birdpress_hero_ratio":"","_birdpress_hero_h1":"","_birdpress_hero_media_id":0,"_birdpress_hero_media_array_id":[],"_birdpress_hero_media_array":[],"_birdpress_hero_media":0,"_birdpress_hero_video_id":0,"_birdpress_hero_video":0,"_birdpress_hero_youtube":"","_birdpress_hero_content":true,"_birdpress_hero_byline":"","_birdpress_hero_byline_bottom":"","_birdpress_hero_button_link":"","_birdpress_hero_button_text":"","_birdpress_hero_button_color":"","_birdpress_hero_date":false,"original_guid":"","_birdpress_hide_search":false,"_birdpress_page_width":"","_birdpress_global_cta":false,"_birdpress_widget_sidebar":"","_birdpress_next_article":0,"_birdpress_next_article_title":"","_birdpress_prev_article":0,"_birdpress_prev_article_title":"","_birdpress_sub_navigation_id":0,"_birdpress_sub_navigation":"","_birdpress_sub_navigation_title":false,"_birdpress_anchor_navigation_id":0,"_birdpress_anchor_navigation":"","_birdpress_postType":"both","_birdpress_categoryID":0,"_birdpress_tagID":0,"_birdpress_parentPostID":0,"_birdpress_parentPostTitle":"","_birdpress_menuID":0,"_birdpress_menuName":"","_birdpress_listHeader":"","_birdpress_listLayout":"card-display","_birdpress_listColumns":"","_birdpress_maxItems":12,"_birdpress_listPaginate":true,"_birdpress_displaySort":true,"_birdpress_sortOrder":"DESC","_birdpress_sortBy":"date","_birdpress_listID":"","_birdpress_listClass":"","_birdpress_displayImages":true,"_birdpress_displayCaptions":false,"_birdpress_displayExcerpts":false,"_birdpress_attTop":"","_birdpress_attBottom":"","_birdpress_showLogos":false,"_birdpress_post_logo":0,"footnotes":""},"categories":[29,16,1],"tags":[],"content-format":[],"class_list":["post-5604","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-blog","category-news","category-uncategorized"],"_links":{"self":[{"href":"https:\/\/www.birds.cornell.edu\/ccb\/wp-json\/wp\/v2\/posts\/5604","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.birds.cornell.edu\/ccb\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.birds.cornell.edu\/ccb\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.birds.cornell.edu\/ccb\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.birds.cornell.edu\/ccb\/wp-json\/wp\/v2\/comments?post=5604"}],"version-history":[{"count":0,"href":"https:\/\/www.birds.cornell.edu\/ccb\/wp-json\/wp\/v2\/posts\/5604\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.birds.cornell.edu\/ccb\/wp-json\/wp\/v2\/media\/5717"}],"wp:attachment":[{"href":"https:\/\/www.birds.cornell.edu\/ccb\/wp-json\/wp\/v2\/media?parent=5604"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.birds.cornell.edu\/ccb\/wp-json\/wp\/v2\/categories?post=5604"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.birds.cornell.edu\/ccb\/wp-json\/wp\/v2\/tags?post=5604"},{"taxonomy":"content-format","embeddable":true,"href":"https:\/\/www.birds.cornell.edu\/ccb\/wp-json\/wp\/v2\/content-format?post=5604"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}